ligand
p-aminobenzamidine
包装
pack of 5 × 1 mL
制造商/商品名称
Cytiva 17-5143-01
储存条件
(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
参数
42 psi
柱床尺寸
7 mm × 25 mm
柱床体积
1 mL
色谱柱内径
7 mm
基质
highly cross-linked 4% agarose
平均直径
90 μm
cleaning
1-9
工作范围
2-8
储存温度
2-8°C
一般描述
应用
The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System.
特点和优势
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
- Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
- HiTrap™ Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
- High binding capacity.
- The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA™ design or FPLC™ System.
制备说明
分析说明
法律信息
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
商品
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
实验方案
Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.
Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
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