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Merck
CN

GE17-5158-01

HiTrap® Q XL

Cytiva 17-5158-01, pack of 5 × 1 mL

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UNSPSC代码:
23151816
NACRES:
NA.56

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ligand

quaternary amine

描述

Ion Exchanger Type (value)

表单

prepacked column

交联

cross-linked

包装

pack of 5 × 1 mL

制造商/商品名称

Cytiva 17-5158-01

储存条件

(20% Ethanol)

参数

-70 psi max. pressure (max flow rate = 20 ml/min)

技术

liquid chromatography (LC): suitable

柱床尺寸

7 mm × 25 mm

柱床体积

1 mL

色谱柱内径

7 mm

基质

6% cross-linked agarose with dextran surface extenders

基质活性基团

Q functional group, ammonium group

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14

工作范围

2-12

容量

binding capacity(>= 160 mg albumin / mL)
 ion exchange capacity(0.18-0.26 mmol Cl / mL)

适用性

suitable for bioprocess medium

相关类别

一般描述

HiTrap® Q XL are strong anion exchangers prepacked with Q Sepharose™ XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

应用

Q Sepharose™ XL and SP Sepharose™ XL media have long chains of Dextran coupled to a robust, 6% highly cross-linked Agarose matrix. The Dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.

特点和优势

  • High binding capacities with high fLow rates.
  • Convenient and affordable for fast, easy separations either alone or connected in series.
  • Designed for use with syringe, peristaltic pump, or chromatography system such as ÄKTA® design.

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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商品

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

实验方案

Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up

This page covers how and when to use Sepharose XL media for purification of proteins.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from Cytiva.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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