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Merck
CN

GE17-5158-01

HiTrap® Q XL

Cytiva 17-5158-01, pack of 5 × 1 mL

别名:

IEX column, Q Sepharose XL, chromatography cartridge, cytiva 17-5158-01, ion exchange resin, prepacked column, strong anion exchanger

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NACRES:
NA.56
UNSPSC Code:
23151816

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ligand

quaternary amine

description

Ion Exchanger Type (value)

form

prepacked column

crosslinking

cross-linked

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 17-5158-01

storage condition

(20% Ethanol)

parameter

-70 psi max. pressure (max flow rate = 20 ml/min)

technique(s)

liquid chromatography (LC): suitable

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

6% cross-linked agarose with dextran surface extenders

matrix active group

Q functional group, ammonium group

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14

working range

2-12

capacity

binding capacity(>= 160 mg albumin / mL), ion exchange capacity(0.18-0.26 mmol Cl / mL)

suitability

suitable for bioprocess medium

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General description

HiTrap® Q XL are strong anion exchangers prepacked with Q Sepharose™ XL media, optimized for fast, convenient small-scale protein capture using ion exchange (IEX) chromatography.

Application

Q Sepharose™ XL and SP Sepharose™ XL media have long chains of Dextran coupled to a robust, 6% highly cross-linked Agarose matrix. The Dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities.

Features and Benefits

  • High binding capacities with high fLow rates.
  • Convenient and affordable for fast, easy separations either alone or connected in series.
  • Designed for use with syringe, peristaltic pump, or chromatography system such as ÄKTA® design.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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商品

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

实验方案

Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up

This page covers how and when to use Sepharose XL media for purification of proteins.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from Cytiva.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

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