material
bed support (polyamide), glass column, polypropylene fiber (reinforced glass)
product line
Superdex®
mol wt
3000-70000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 17-5174-01
parameter
0.5 ml/min - 1 ml/min flow rate, 1.8 Mpa (261 psi), 25-500 μL sample volume
bed volume
24 mL
column L × I.D.
30 cm × 10 mm
matrix
agarose-dextran composite, polymeric
avg. part. size
13 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
General description
Superdex® 75 10/300 GL is a prepacked gel filtration column for high-resolution, semipreparative, and analytical separations of biomolecules. Superdex® 75 has a separation range for molecules with molecular weights between 3 000 and 70 000.
Superdex® prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
Superdex® 75 5/150 GL and Superdex® 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Superdex® columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn Column Family excellent reproducibility and durability in purification using a chromatography system.
Superdex® prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
Superdex® 75 5/150 GL and Superdex® 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Superdex® columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn Column Family excellent reproducibility and durability in purification using a chromatography system.
Application
Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place)
for high-resolution, semipreperative and analytical separations of biomolecules
Features and Benefits
- High-resolution separations of proteins, peptides, and other biomolecules according to size.
- Well suited for the polishing step in a purification procedure.
- Separations in two ranges from Mr 3000 up to 600 000 (globular proteins).
- Easy and predictable scale-up.
- Excellent reproducibility and durability.
- For rapid protein homogeneity analysis or purity check use the 5/150 GL short columns.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Superdex is a registered trademark of Cytiva
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Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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