ligand
butyl
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-5197-01
parameter
<20 mL/min flow rate, 42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
4% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
3-13
suitability
suitable for bioprocess medium
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General description
Features and Benefits
- Provide convenient and fast small-scale Protein purifications.
- Butyl Sepharose™ 4 Fast FLow is a well-established, standard aliphatic HIC medium for capture and intermediate purification.
- Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA® design.
- High fLow rates and high capacities.
- Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.
Preparation Note
Analysis Note
Other Notes
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
Optimize salt conditions for protein binding and impurity passage in chromatography to achieve required selectivity.
Column, media, and sample preparation for Hydrophobic Interaction Chromatography (HIC) detailed with Cytiva media.
This page discusses column packing and preparation techniques for reverse phase chromatography.
实验方案
This page discusses various aspects of sample preparation for chromatographic purification.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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