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GE17-5279-02

Glutathione Seph High Performance

Name: Glutathione Seph High Performance
Brand: SIGMA

Cytiva 17-5279-02, pack of 100 mL

别名:

FPLC column packing, GST affinity matrix, affinity resin, glutathione s-transferase affinity polymer, stationary phase

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关于此项目

NACRES:

NA.56

UNSPSC Code:

23151817

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属性

ligand

glutathione

packaging

pack of 100 mL

manufacturer/tradename

Cytiva 17-5279-02

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

3-12

working range

3-12

capacity

>7 mg/mL binding capacity(medium)

说明

General description

Glutathione Sepharose^™ High Performance is an affinity medium for high resolution purification of Glutathione S transferase (GST) tagged proteins.

Application

Glutathione Sepharose^™ High Performance is used for the purification of GST-tagged Proteins from bacterial lysates and other S-transferases or glutathione-dependent Proteins. GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function.

Features and Benefits

34 μm bead size for improved efficiency and resolution
Fast, one-step purification of GST-tagged Proteins and other glutathione S-trasferase or glutathione-dependent Proteins
Mild conditions during the purification process preserves Protein antigenicity and function

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

GHS02

signalword

Warning

hcodes

H226

pcodes

P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501

存储类别

3 - Flammable liquids

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

GSTrap™ Columns for Purification

GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

商品

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

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全球贸易项目编号

货号	GTIN
GE17-5279-02	04061837622137