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Merck
CN

GE17-5282-01

GSTrap™ High Performance

Cytiva 17-5282-01, pack of 1 × 5 mL

别名:

GSTrap Column

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NACRES:
NA.56
UNSPSC Code:
23151816

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ligand

glutathione

packaging

pack of 1 × 5 mL

manufacturer/tradename

Cytiva 17-5282-01

parameter

flow rate, <20 mL/min flow rate, 42 psi (H2O at room temperature.)

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

3-12

working range

3-12

capacity

>7 mg/mL binding capacity (medium)

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General description

GSTrap™ HP columns are prepacked with Glutathione Sepharose™ High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Application

GSTrap™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged Proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose™ High Performance. GST-tagged Proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.

GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods.

Features and Benefits

  • High resolution purification of GST-tagged Proteins.
  • Mild elution conditions preserving Protein antigenicity and function.
  • Fast, one-step purification.
  • Easy to use with syringe, pump or a chromatography system.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

wgk

WGK 1

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商品

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

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实验方案

GSTrap™ Columns for Purification

GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

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