产品名称
Ni Sepharose™ 6Fast Flow, Cytiva 17-5318-01, pack of 25 mL
form
resin
crosslinking
cross-linked
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-5318-01
storage condition
(20% ethanol)
parameter
150 cm/hr flow rate
technique(s)
liquid chromatography (LC): suitable
matrix
6% cross-linked agarose
matrix active group
Ni2+ ions
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
~40 mg binding capacity(histidine-tagged protein)
separation technique
affinity
shipped in
ambient
storage temp.
2-30°C
Analysis Note
Features and Benefits
- Designed for scaling up purification of histidine-tagged proteins
- High protein binding capacity and minimal leakage of Ni2? ions
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in convenient prepacked HisPrep™ and HisTrap™ column formats.
General description
Ni Sepharose™ 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.
Ni Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity.
Ni Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Ni Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Legal Information
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Preparation Note
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
实验方案
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from Cytiva.
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