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Merck
CN

GE17-5318-02

Ni Sepharose 6Fast Flow

Cytiva 17-5318-02, pack of 100 mL

别名:

Ni-Sepharose Fast Flow, Nickel Affinity Resin, Nickel Column

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NACRES:
NA.56
UNSPSC Code:
41106500
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form

resin ()

crosslinking

cross-linked

packaging

pack of 100 mL

manufacturer/tradename

Cytiva 17-5318-02

storage condition

(20% Ethanol)

parameter

150 cm/hr flow rate

technique(s)

liquid chromatography (LC): suitable

matrix

6% cross-linked agarose

matrix active group

Ni2+ ions

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

~40 mg binding capacity (histidine-tagged protein / mL resin)

separation technique

affinity

shipped in

ambient

storage temp.

2-30°C

General description

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity..

Ni Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As A member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Designed for scaling up purification of histidine-tagged proteins
  • High protein binding capacity and minimal leakage of Ni2? ions
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.

Preparation Note

Storage Temp.: 4°C to 30°C
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva


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存储类别

3 - Flammable liquids

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实验方案

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from Cytiva.

Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.

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商品

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

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全球贸易项目编号

货号GTIN
GE17-5318-0204061837622274