ligand
recombinant protein L (E. coli), mammalian free
packaging
pack of 200 mL
manufacturer/tradename
Cytiva 17-5478-02
storage condition
(20% Ehtanol)
matrix
rigid, highly cross-linked agarose
avg. part. size
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
working range
2-10(pH interval where the medium can be operated without significant change in function.)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
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General description
Capto™ L resin is designed for antibody fragment purification, when the fragment contains the kappa light chain.
Affinity resin for antibody fragment purification
Capto™ L combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains. The high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody (dAb), scFv, and Fab purification.
Using this protein L resin
Capto™ L resin requires minimal optimization and produces high purity in one separation step, resulting in significant savings during process development. This resin is also advantageous in manufacturing, because high selectivity of affinity capture reduces the number of subsequent polishing steps.
Affinity resin for antibody fragment purification
Capto™ L combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains. The high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody (dAb), scFv, and Fab purification.
Using this protein L resin
Capto™ L resin requires minimal optimization and produces high purity in one separation step, resulting in significant savings during process development. This resin is also advantageous in manufacturing, because high selectivity of affinity capture reduces the number of subsequent polishing steps.
Application
Purification of antibody fragments using affinity chromatography
Features and Benefits
- Suitable for the capture of a wide range of antibody fragments such as Fabs, dAbs, and single-chain fragment variable (scFv) .
- High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
- Enables a platform approach to antibody fragment purification.Excellent productivity, process economy, and process design flexibility due to rigid matrix.
- Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
- Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Capto is a trademark of Cytiva
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Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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实验方案
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
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