ligand
recombinant protein L (E. coli), mammalian free
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-5478-55
parameter
5 bar
column I.D.
16 mm
matrix
rigid, highly cross-linked agarose
avg. part. size
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
working range
2-10(pH interval where the medium can be operated without significant change in function.)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
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General description
HiTrap® Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.
Features and Benefits
- Suitable for the capture of a wide range of antibody fragments such as antigen-binding fragments (Fabs), domain antibodies (dAbs), and single-chain variable fragments (scFvs).
- Enables a platform approach to antibody fragment purification.
- High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
- Convenient for process development, as well as small-scale purification.
- HiTrap™ format enables easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA™ system.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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实验方案
This page shows how to purify antibody fragments by affinity chromatography using Capto L products from Cytiva.
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