form
liquid
feature
wetted part: no
packaging
pkg of 1 mL
manufacturer/tradename
Cytiva 17-6003-18
storage condition
(Rehydration Solution -20)
color
Colorless
storage temp.
2-8°C
General description
DeStreak™ Reagent.
Application
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of Proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved Protein patterns and reduced reproducibility between electrophoresis runs.
DeStreak™ Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak™ Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.
DeStreak™ Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak™ Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak™ Reagent is also available separately in a 1-ml pack size.
DeStreak™ Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak™ Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.
DeStreak™ Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak™ Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak™ Reagent is also available separately in a 1-ml pack size.
Features and Benefits
- Improves reproducibility and quality of 2-D gels by preventing streaking.
- Eliminates extra spots caused by nonspecific oxidation of Proteins.
- Stabilizes Proteins throughout entire 2-D runs, ensuring simplified, well-resolved Protein patterns.
- Highly suitable for both analytical and preparative gels.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
DeStreak is a trademark of Cytiva
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral - Eye Dam. 1
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
233.6 °F - closed cup
flash_point_c
112 °C - closed cup
法规信息
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商品
This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.
实验方案
This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
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