产品名称
PreScission Protease, Cytiva 27-0843-01
packaging
pkg of 500 units
manufacturer/tradename
Cytiva 27-0843-01
shipped in
dry ice
storage temp.
−70°C
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Application
Specific, low temperature cleavage of fusion protein tags; Simple on-column cleavage during affinity purification
PreScission Protease has been used for eluting GST-tagged AT3, during in vitro ubiquitination reactions and AT3-Ub preparation. It has also been used to cleave GST tag from the a-enolase during induction of GST a-enolase expression in the protease-deficient BL21 strain of Escherichia coli.
PreScission Protease has been used for eluting GST-tagged AT3, during in vitro ubiquitination reactions and AT3-Ub preparation. It has also been used to cleave GST tag from the a-enolase during induction of GST a-enolase expression in the protease-deficient BL21 strain of Escherichia coli.
Features and Benefits
- Specific cleavage – between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro.
- Time-saving – GST-tagged proteins can be cleaved while still bound to an affinity resin.
- Low incubation temperature – enables low temperature cleavage of fusion proteins containing the HRV 3C protease recognition sequence.
General description
PreScission Protease is a fusion protein of human rhinovirus (HRV) 3C protease and GST. It allows for on-column cleavage of GST tags and protein purification in one step.
On-column or off-column enzyme cleavage
GST tags and protease can be removed in a single step while fusion proteins are still bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow affinity resin. Another option is to elute the GST-tagged protein using reduced glutathione, cleave, then pass the sample over the same resin to remove the protease and tags. Unlike other common proteases, such as thrombin and Factor Xa, this GST-tagged fusion protein can be removed easily through binding to a resin with affinity for GST. Other non-recombinant proteases require an additional purification step with a different resin.
Vectors encoding the HRV 3C protease recognition sequence
pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 encode the recognition sequence for this 3C protease.
On-column or off-column enzyme cleavage
GST tags and protease can be removed in a single step while fusion proteins are still bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow affinity resin. Another option is to elute the GST-tagged protein using reduced glutathione, cleave, then pass the sample over the same resin to remove the protease and tags. Unlike other common proteases, such as thrombin and Factor Xa, this GST-tagged fusion protein can be removed easily through binding to a resin with affinity for GST. Other non-recombinant proteases require an additional purification step with a different resin.
Vectors encoding the HRV 3C protease recognition sequence
pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 encode the recognition sequence for this 3C protease.
Legal Information
pGEX Vectors
pGEX Vectors are to be used for scientific investigation and research and for no other purpose whatsoever and a license for commercial use of the licensed products and the processes claimed in US patent 5,654,176 and equivalent patents and patent applications in other countries must be negotiated directly with Millipore Corp (formerly Chemicon International Inc) by the purchaser prior to such use.
pGEX Vectors are to be used for scientific investigation and research and for no other purpose whatsoever and a license for commercial use of the licensed products and the processes claimed in US patent 5,654,176 and equivalent patents and patent applications in other countries must be negotiated directly with Millipore Corp (formerly Chemicon International Inc) by the purchaser prior to such use.
Preparation Note
Storage Condition: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials.
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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商品
This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
实验方案
Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.
This page describes the characteristics of pGEX expression vectors used with Cytiva products.
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