包装
pack of 1 kit
制造商/商品名称
Cytiva 27-4570-01
运输
wet ice
储存温度
2-8°C
一般描述
GST bulk kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins.
应用
GST Bulk Kit contains a 10 mL bulk pack of Glutathione Sepharose™ 4B medium, five gravity-fLow columns, IPTG, and GST Buffer Kit. GST-tagged Proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of Glutathione Sepharose™ 4B, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used.
特点和优势
- For rapid purification of GST-tagged Proteins using gravity flow columns or using batch purification with high specificity.
- Preserves Protein antigenicity and function.
- Yields up to 50 mg of pure Protein.
制备说明
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
法规信息
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商品
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
实验方案
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
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