产品名称
GST Bulk Kit, Cytiva 27-4570-01, pack of 1 kit
packaging
pack of 1 kit
manufacturer/tradename
Cytiva 27-4570-01
shipped in
wet ice
storage temp.
2-8°C
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Application
GST Bulk Kit contains a 10 mL bulk pack of Glutathione Sepharose™ 4B medium, five gravity-fLow columns, IPTG, and GST Buffer Kit. GST-tagged Proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of Glutathione Sepharose™ 4B, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used.
Features and Benefits
- For rapid purification of GST-tagged Proteins using gravity flow columns or using batch purification with high specificity.
- Preserves Protein antigenicity and function.
- Yields up to 50 mg of pure Protein.
General description
GST bulk kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Legal Information
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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商品
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
实验方案
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
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