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Merck
CN

GE27-5325-01

Microspin™ G-25 Columns

Cytiva 27-5325-01

别名:

G-25, sephadex, spin columns, DNA filters, PCR cleanup tube

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NACRES:
NA.52
UNSPSC Code:
41105501

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产品名称

Microspin™ G-25 Columns, Cytiva 27-5325-01

material

resin (Sephadex G-25 DNA grade F)

sterility

non-sterile

manufacturer/tradename

Cytiva 27-5325-01

packaging

pkg of 50 columns

working volume

10 —100 μL

particle size

20-80 μm (dry)

pore size

5000  Da MWCO

working range

2-13

相关类别

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

  • For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin-column chromatography.
  • Excellent for rapid purification of newly synthesized oligonucleotides >10-mers in 100 to 150 μl of deprotection solution using spin-column chromatography.
  • Convenient: Prepacked with Sephadex® G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
  • Ready to use: Requires less than 4 min from sample application to collection of purified product.
  • Tested in nickase, single and double-stranded exonuclease and RNase assays.
  • Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 μl.

General description

MicroSpin™ G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.

MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 20 to 25 °C. (Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative)

Legal Information

MicroSpin is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

存储类别

12 - Non Combustible Liquids

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