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NACRES:
NA.52
UNSPSC Code:
41105501
Material:
resin (Sephadex G-25 DNA grade F)
Pore size:
5000 Da MWCO
Working volume:
10 —100 μL
Sterility:
non-sterile
Packaging:
pkg of 50 columns
material
resin (Sephadex G-25 DNA grade F)
sterility
non-sterile
manufacturer/tradename
Cytiva 27-5325-01
packaging
pkg of 50 columns
working volume
10 —100 μL
particle size
20-80 μm (dry)
pore size
5000 Da MWCO
working range
2-13
相关类别
General description
MicroSpin™ G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.
MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.
MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.
Features and Benefits
- For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin-column chromatography.
- Excellent for rapid purification of newly synthesized oligonucleotides >10-mers in 100 to 150 μl of deprotection solution using spin-column chromatography.
- Convenient: Prepacked with Sephadex® G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
- Ready to use: Requires less than 4 min from sample application to collection of purified product.
- Tested in nickase, single and double-stranded exonuclease and RNase assays.
- Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 μl.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 20 to 25 °C. (Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
MicroSpin is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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