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Merck
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GE27-5330-02

Microspin™ G-50 Columns

Cytiva 27-5330-02, pack of 250 columns

别名:

Microspin columns, Amersham spin column, DNA purification columns, PCR cleanup columns, size exclusion columns, spin column

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NACRES:
NA.52
UNSPSC Code:
41105501
Material:
resin (Sephadex G-50 DNA Grade)
Pore size:
30 kDa MWCO
Working volume:
12—50 μL
Sterility:
non-sterile
Packaging:
pack of 250 columns

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material

resin (Sephadex G-50 DNA Grade)

sterility

non-sterile

packaging

pack of 250 columns

manufacturer/tradename

Cytiva 27-5330-02

working volume

12—50 μL

particle size

50-150 μm (dry)

pore size

30 kDa MWCO

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General description

MicroSpin™ G-50 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions. Good product yield and purity is obtained with sample volumes from 12-50 ul. They are suitable for any DNA greater than 20 bases in length. They will not remove or denature enzyme.

Features and Benefits

  • For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μ using spin-column chromatography.
  • Columns are prepacked with Sephadex® G-50 Grade pre-equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide.
  • Product recovery will be at least 10% lower than that from ProbeQuant™ G-50 Micro Columns.
  • Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 μ) following heat denaturation and phenol extraction.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 20 to 25 °C. (TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

MicroSpin is a trademark of Cytiva
ProbeQuant is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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Domagoj Baretić et al.
Molecular cell, 78(5), 926-940 (2020-05-06)
The eukaryotic replisome, organized around the Cdc45-MCM-GINS (CMG) helicase, orchestrates chromosome replication. Multiple factors associate directly with CMG, including Ctf4 and the heterotrimeric fork protection complex (Csm3/Tof1 and Mrc1), which has important roles including aiding normal replication rates and stabilizing

实验方案

DNA Cleanup using illustra™ GFX Kits

DNA cleanup using illustra™ GFX kits from Cytiva.

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