物料
resin (Sephadex G-50 DNA Grade)
无菌性
non-sterile
包装
pack of 250 columns
制造商/商品名称
Cytiva 27-5330-02
工作体积
12—50 μL
粒径
50-150 μm (dry)
孔径
30 kDa MWCO
相关类别
一般描述
MicroSpin™ G-50 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions. Good product yield and purity is obtained with sample volumes from 12-50 ul. They are suitable for any DNA greater than 20 bases in length. They will not remove or denature enzyme.
特点和优势
- For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μ using spin-column chromatography.
- Columns are prepacked with Sephadex® G-50 Grade pre-equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide.
- Product recovery will be at least 10% lower than that from ProbeQuant™ G-50 Micro Columns.
- Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 μ) following heat denaturation and phenol extraction.
制备说明
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 20 to 25 °C. (TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
MicroSpin is a trademark of Cytiva
ProbeQuant is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva
储存分类代码
12 - Non Combustible Liquids
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