物料
polypropylene and polyethylene
包装
pack of 4 plates
制造商/商品名称
Cytiva 28-4009-90
尺寸
127.8 mm × 85.5 mm × 30.6 mm
基质
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14(Ni2+-stripped medium.)
工作范围
3-12(Ni2+-stripped medium.)
容量
~40 mg binding capacity(histidine-tagged protein)
适用性
suitable for bioprocess medium
一般描述
His MultiTrap™ FF and His MultiTrap™ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).
应用
His MultiTrap™ FF and His MultiTrap™ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose™ 6 Fast FLow (His MultiTrap™ FF) or Ni Sepharose™ High Performance (His MultiTrap™ HP). A protocol for His MultiTrap™ is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.
Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap™ FF and His MultiTrap™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.
Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap™ FF and His MultiTrap™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.
特点和优势
- Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.
- Increased convenience and consistency with prepacked 96-well plates.
- No filtration needed - load unclarified sample directly, increase reproducibility, and save time.
- Use His MultiTrap™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.
- Easy scale-up to HisTrap™ FF, HisPrep™ FF 16/10 or HisTrap™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.
制备说明
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
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