跳转至内容
Merck
CN

GE28-4055-00

GST MultiTrap 4B

Cytiva 28-4055-00

别名:

GST-tagged purification system, chromatography plate, filter plate, glutathione Sepharose 4B, prepacked affinity plate

登录 查看组织和合同定价。

选择尺寸


关于此项目

NACRES:
NA.32
UNSPSC Code:
12161501
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

ligand

glutathione

material

polypropylene and polyethylene

manufacturer/tradename

Cytiva 28-4055-00

packaging

pkg of 4 x 96-well

size

127.8 mm × 85.5 mm × 30.6 mm

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

4-13

working range

4-13

capacity

>25 mg binding capacity (horse liver GST/ml medium)

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.

Application

GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.

Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.

GST MultiTrap FF is prepacked with Glutathione Sepharose 4 Fast Flow, while GST MultiTrap 4B is prepacked with Glutathione Sepharose 4B.

Features and Benefits

  • 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
  • Highly reproducible well-to well and plate-to-plate results.
  • High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
  • Wells are prepacked with Glutathione Sepharose media for convenience.
  • Easy and predictable scale-up using prepacked GSTrap FF, GSTPrep FF 16/10, and GSTrap 4B columns.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GSTPrep is a trademark of Cytiva
GSTrap is a trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

存储类别

3 - Flammable liquids

法规信息

新产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助,请联系 客户支持

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

商品

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

实验方案

This page shows how to solubilize membrane proteins with products from Cytiva.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持