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Merck
CN

GE28-4082-55

HiTrap® MabSelect™

Cytiva 28-4082-55, pack of 1 × 5 mL

别名:

affinity column, chromatography column, mAb purification column, prepacked column, protein A column

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UNSPSC代码:
23151816
NACRES:
NA.56

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ligand

recombinant protein A (E. coli)

包装

pack of 1 × 5 mL

制造商/商品名称

Cytiva 28-4082-55

储存条件

(20% Ehtanol)
 (20% Ethanol)

参数

<20 mL/min flow rate
42 psi

柱床尺寸

16 mm × 25 mm

柱床体积

5 mL

色谱柱内径

16 mm

基质

highly cross-linked agarose

平均零件尺寸

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-12

工作范围

3-10

容量

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

适用性

suitable for bioprocess medium

储存温度

2-8°C

相关类别

一般描述

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect™ columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

应用

Lab Purification using the MabSelect™ Protein A tag for capture of antibodies.

特点和优势

  • HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

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Lot/Batch Number

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商品

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

实验方案

Protein A-Based Separation with Cytiva Products

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

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