ligand
recombinant protein A (E. coli)
包装
pack of 1 × 5 mL
制造商/商品名称
Cytiva 28-4082-55
储存条件
(20% Ehtanol)
(20% Ethanol)
参数
<20 mL/min flow rate
42 psi
柱床尺寸
16 mm × 25 mm
柱床体积
5 mL
色谱柱内径
16 mm
基质
highly cross-linked agarose
平均零件尺寸
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-12
工作范围
3-10
容量
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
适用性
suitable for bioprocess medium
储存温度
2-8°C
一般描述
Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect™ columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
应用
Lab Purification using the MabSelect™ Protein A tag for capture of antibodies.
特点和优势
- HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
其他说明
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法律信息
HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
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