ligand
native protein A
特点
wetted part: no
包装
pack of 16 columns
制造商/商品名称
Cytiva 28-9031-32
储存条件
(20% Ethanol)
基质
highly cross-linked 6% agarose
平均零件尺寸
34 μm
cleaning
2-10
工作范围
3-9
容量
>1 mg binding capacity(human IgG well)
储存温度
2-8°C
一般描述
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
protein A HP SpinTrap™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose™ HP.
应用
Protein A Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
特点和优势
- For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use.
- Classic and cross-link protocols provide flexibility.
- Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
- Fast binding kinetics and high capacity provide high yield.
- Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- Easy scale-up with HiTrap® Protein A HP prepacked columns.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva
警示用语：
Warning
危险声明
储存分类代码
3 - Flammable liquids
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