ligand
native protein A
feature
wetted part: no
packaging
pack of 16 columns
manufacturer/tradename
Cytiva 28-9031-32
storage condition
(20% Ethanol)
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-10
working range
3-9
capacity
>1 mg binding capacity(human IgG well)
storage temp.
2-8°C
General description
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
protein A HP SpinTrap™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose™ HP.
Application
Protein A Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
Features and Benefits
- For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use.
- Classic and cross-link protocols provide flexibility.
- Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
- Fast binding kinetics and high capacity provide high yield.
- Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- Easy scale-up with HiTrap® Protein A HP prepacked columns.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
新产品
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实验方案
This page shows how to separate IgG antibodies by affinity chromatography using Protein A HP SpinTrap columns from Cytiva.
This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.
商品
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| GE28-9031-32 | 04061834084204 |