separation technique
affinity
material
polypropylene and polyethylene plate
form
plate (prepacked)
crosslinking
cross-linked
packaging
pkg of 4 ea
manufacturer/tradename
Cytiva 28-9031-33
storage condition
(20% Ethanol)
parameter
-0.5 bar vacuum limit, 700 × g max. centrifugal force
technique(s)
liquid chromatography (LC): suitable
L × W × H
127.8 mm × 85.5 mm × 30.6 mm
bed size
50 μL , per well
well volume
800 μL
matrix
highly cross-linked 6% agarose
matrix active group
native protein A
particle size
~34 μm
operating pH
3-9
capacity
>10 mg binding capacity (human IgG / mL medium)(Human IgG)
storage temp.
2-30°C
General description
Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species.
Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
Protein A Sepharose™ High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Features and Benefits
- Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
- Flexible format, with protocols for classic elution and cross-link method.
- Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
- Fast binding kinetics and high capacity provides high yield.
- Easy scale-up with HiTrap® Protein A HP prepacked columns.
- Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- MultiTrap™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiTrap is a registered trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
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