ligand
diethylaminoethyl
description
Ion Exchanger Type (value)
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 28-9165-40
parameter
<20 mL/min flow rate, 42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked agarose with dextran surface extender
average diameter
90 μm (d50v)
cleaning
2-14
working range
2-12
suitability
suitable for bioprocess medium
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General description
HiTrap Capto DEAE is a weak anion exchanger prepacked with BioProcess Capto resins for screening and small-scale protein purifications using ion exchange chromatography (IEX).
Capto DEAE has a weak diethylaminoethyl anion exchanger coupled to a chemically modified, high-flow agarose matrix. The modified agarose matrix provides greater particle rigidity without compromising pore size, which allows more flexibility in process-scale applications. High dynamic binding capacity is achieved with a dextran surface extender that coats the agarose matrix.
Capto DEAE has a weak diethylaminoethyl anion exchanger coupled to a chemically modified, high-flow agarose matrix. The modified agarose matrix provides greater particle rigidity without compromising pore size, which allows more flexibility in process-scale applications. High dynamic binding capacity is achieved with a dextran surface extender that coats the agarose matrix.
Features and Benefits
- High-throughput resin for capture and intermediate purification, excellent choice for process-scale purification.
- The combined properties of high binding capacity, high flow rate, and low back pressure reduces process cycle times and improves productivity at process scale.
- Rigid agarose matrix enables high bed heights and purification of viscous samples at high flow rates.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
Capto is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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实验方案
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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