separation technique
affinity
storage temp.
2-8°C
form
resin
crosslinking
cross-linked
packaging
pack of 1 × 5 mL
manufacturer/tradename
Cytiva 28-9187-79
storage condition
(20% ethanol)
parameter
0.3 Mpa max. pressure, 20 mL/min max. flow rate, 5 mL/min flow rate (recommended)
technique(s)
liquid chromatography (LC): suitable
bed volume
5 mL
column I.D. × L
16 mm × 25 mm
matrix
highly cross-linked 6% agarose
matrix active group
dextrin
avg. part. size
34 μm
cleaning
2-13
operating pH
>7
capacity
~16 mg binding capacity (MBP2*-b-galactosidase / mL medium), ~7 mg binding capacity (MBP2*-paramyosin-d-Sal / mL medium)
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General description
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
Features and Benefits
- HiTrap™ columns prepacked with Dextrin Sepharose™ High Performance medium.
- Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
- Physiological conditions and mild elution preserve the activity of the target protein.
- Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.
Preparation Note
Analysis Note
Other Notes
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products.
实验方案
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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