material
polypropylene (PP)
mol wt
40000-20000000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9356-07
parameter
<1.3 mL/min flow rate, <13 mL sample volume, 0.15 Mpa (22 psi)
bed volume
320 mL
column L × I.D.
60 cm × 26 mm
matrix
dextran-acrylamide particle platform, allyl dextran and N, N’-methylenebisacrylamide, polymeric
matrix active group
dextran-acrylamide phase
avg. part. size
50 μm
cleaning
2-13
working range
3-11
separation technique
size exclusion (SEC)
General description
HiPrep 26/60 Sephacryl S-500 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl S-500 is used for separating polysaccarides, macromolecules with extended structures, and even small plasmids.
Application
All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
gel filtration columns for low to medium pressure ÄKTA systems+AE14
Features and Benefits
- Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
- Gel filtration columns for low to medium pressure ÄKTA systems.
- Well-established in industrial production.
- The HiPrep Sephacryl series offers reproducible separations over a wide molecular weight range.
- Convenient, prepacked HiPrep Sephacryl columns speed up protein purification.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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实验方案
Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.
本页介绍如何使用Cytiva医疗的sephacryl色谱柱进行分离，该色谱柱是通过将烯丙基葡聚糖与N,N'-亚甲基双丙烯酰胺共价交联而制备的尺寸排阻色谱介质。
相关内容
Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.
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