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Merck
CN

GE28-9365-40

HiPrep™ SP XL 16/10

Cytiva 28-9365-40

别名:

IEX column, SP Sepharose XL, chromatography cartridge, cytiva 28-9365-40, ion exchange resin, prepacked column, strong cation exchanger

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UNSPSC代码:
47101511
NACRES:
NA.56

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ligand

sulphopropyl

描述

Ion Exchanger Type (value)

包装

pkg of 20 mL

制造商/商品名称

Cytiva 28-9365-40

参数

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

柱床尺寸

16 mm × 100 mm

柱床体积

20 mL

色谱柱内径

16 mm

基质

6% cross-linked agarose with dextran surface extenders

粒径

45-165 μm

平均直径

90 μm

cleaning

3-14

工作范围

4-13

适用性

suitable for bioprocess medium

相关类别

一般描述

HiPrep™ SP XL 16/10 is a strong cation exchanger prepacked with Q Sepharose™ XL columns for inital capture of proteins and other biomoleculs with ion exchange (IEX) chromatography.

应用

HiPrep™ SP XL 16/10 is a prepacked, ready to use column for ion exchange chromatography. It provides fast, preparative separations of Proteins and other biomolecules.

特点和优势

  • Provide high loading capacity and fast purifications.
  • Optimized for reliable, reproducible separations.
  • Compatible with simple pump-based configurations and chromatography systems such as ÄKTA® design.
  • Excellent choice for rapid enrichment during initial capture of Proteins from start material.
  • Strong Sulfopropyl (SP) cation exchanger.

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

HiPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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商品

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

实验方案

Sepharose XL – for selected proteins that require very high binding capacity to increase productivity, easy scale-up

This page covers how and when to use Sepharose XL media for purification of proteins.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

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