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Merck
CN

GE28-9365-41

HiPrep™ DEAE Fast Flow 16/10

Cytiva 28-9365-41

别名:

IEX column, chromatography cartridge, diethylaminoethyl FF, ion exchange column, prepacked column, weak anion exchanger

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UNSPSC代码:
47101511
NACRES:
NA.56

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ligand

diethylaminoethyl

描述

Ion Exchanger Type (value)

包装

pkg of 20 mL

制造商/商品名称

Cytiva 28-9365-41

参数

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

柱床尺寸

16 mm × 100 mm

柱床体积

20 mL

色谱柱内径

16 mm

基质

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14

工作范围

2-12

适用性

suitable for bioprocess medium

相关类别

一般描述

HiPrep™ DEAE FF 16/10 is a weak anion exchanger prepacked with DEAE Sepharose Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography.

HiPrep™ DEAE FF 16/10 is a prepacked, ready to use column for ion exchange chromatography. It provide sfast, preparative separations of proteins and other biomolecules.

特点和优势

  • 20 mL HiPrep™ columns provide fast, reliable, and reproducible preparative ion exchange separations.
  • Good flow rates and high capacity.
  • The industry standard for ion exchange chromatography.
  • Compatible with single-pump configurations and chromatography systems such as AKTA design.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

HiPrep is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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商品

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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实验方案

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

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