ligand
diethylaminoethyl
description
Ion Exchanger Type (value)
packaging
pkg of 20 mL
manufacturer/tradename
Cytiva 28-9365-41
parameter
1.5 bar (22 psi) (Over the Packed Bed During Operation), 2 ml/min - 10 ml/min flow rate, 22 psi
bed size
16 mm × 100 mm
bed volume
20 mL
column I.D.
16 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
2-12
suitability
suitable for bioprocess medium
相关类别
General description
HiPrep™ DEAE FF 16/10 is a prepacked, ready to use column for ion exchange chromatography. It provide sfast, preparative separations of proteins and other biomolecules.
Features and Benefits
- 20 mL HiPrep™ columns provide fast, reliable, and reproducible preparative ion exchange separations.
- Good flow rates and high capacity.
- The industry standard for ion exchange chromatography.
- Compatible with single-pump configurations and chromatography systems such as AKTA design.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
Preparation Note
Analysis Note
Other Notes
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
商品
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
实验方案
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
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