GE28-9365-50

GSTPrep^™ Fast Flow 16/10

Name: GSTPrep<SUP>™</SUP> Fast Flow 16/10
Brand: SIGMA

Cytiva 28-9365-50

别名:

GST-tagged purification columns, affinity column, chromatography cartridge, glutathione Sepharose 4 FF, prepacked column

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NACRES:

NA.56

UNSPSC Code:

13111000

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ligand

glutathione and 10-carbon linker arm

packaging

pkg of 20 mL

manufacturer/tradename

Cytiva 28-9365-50

parameter

<10 mL/min flow rate, 1.5 bar (22 psi) (Over the Packed Bed During Operation), 22 psi

bed size

16 mm × 100 mm

bed volume

20 mL

column I.D.

16 mm

matrix

highly cross-linked 4% agarose

average diameter

90 μm

cleaning

3-12

working range

3-12

capacity

>10 mg/mL binding capacity

General description

GSTPrep^™ FF 16/10 is prepacked, ready-to-use column containing Glutathione Sepharose^™ 4 Fast FLow designed for one-step, scale-up purification of glutathione S-transferase (GST)-tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Application

Glutathione Sepharose^™ 4 Fast FLow is for scale-up purification of recombinant GST-tagged Proteins, other glutathione S-transferases, and glutathione-dependent Proteins. Mild elution conditions preserve Protein antigenicity and function.

Features and Benefits

Fast and easy one-step, scale-up purification of GST-tagged Proteins.
Binds approx. 200 mg recombinant GST/column
Mild elution conditions preserving Protein antigenicity and function.
Simple operation with a pump or chromatography system, such as ÄKTA^® design.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.