ligand
quaternary amine
description
Ion Exchanger Type (value)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9505-10
parameter
1.5 bar (22 psi) (Over the Packed Bed During Operation), 22 psi
bed size
7.7 mm × 100 mm
bed volume
4.7 mL
column I.D.
7.7 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
2-12
suitability
suitable for bioprocess medium
相关类别
General description
HiScreen™ Q FF columns are prepacked with Q Sepharose™ Fast Flow, a strong anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.
Application
The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.
Features and Benefits
- Q Sepharose™ Fast FLow is a strong anion exchanger and IEX Sepharose™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
- Prepacked, ready-to-use columns for convenient process development optimization.
- 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
- Easily connected in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption.
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 2 to 8 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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实验方案
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