ligand
phenyl
form
prepacked column
crosslinking
cross-linked
packaging
pkg of 4.7 mL
manufacturer/tradename
Cytiva 28-9505-16
storage condition
(20% Ethanol)
parameter
44 psi
technique(s)
liquid chromatography (LC): suitable
bed size
7.7 mm × 100 mm
bed volume
4.7 mL
column I.D.
7.7 mm
matrix
6% cross-linked agarose
matrix active group
Phenyl functional group
particle size
24-44 μm
avg. part. size
34 μm
cleaning
2-14
working range
3-13
suitability
suitable for bioprocess medium (BioProcess medium.), suitable for bioprocess medium
separation technique
hydrophobic interaction (HIC)
相关类别
General description
HiScreen™ Phenyl HP columns are prepacked with Phenyl Sepharose™ High Performance hydrophobic interaction chromatography (HIC) media. The columns are an excellent choice for method optimization and parameter screening.
Application
The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen™ HIC columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.
Features and Benefits
- Butyl Sepharose™ High Performance is an aliphatic HIC medium, designed for intermediate and polishing step purification steps when high resolution has priority.
- Prepacked, ready-to-use columns for convenient process development optimization.
- 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
- Easily connected in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption.
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 2 to 8 °C (20% Ethanol or 0.01 M NaOH)
Store at 2 to 8 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
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