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Merck
CN

GE28-9513-79

Protein G Mag Sepharose

Cytiva 28-9513-79, pack of 4 × 500 μL

别名:

affinity capture resin, cytiva 28-9513-79, magnetic pull-down beads, particles, protein G coupled magnetic beads

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UNSPSC代码:
41106500
NACRES:
NA.56
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ligand

protein G

包装

pack of 4 × 500 μL

制造商/商品名称

Cytiva 28-9513-79

储存条件

(20% Ethanol, 20% medium slurry)

参数

Room temperature temp. range

基质

paramagnetic, spherical, highly cross-linked agarose particles

容量

13- 22 mg binding capacity(human IgG/ml medium)

储存温度

2-8°C

一般描述

Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation applications. Based on Sepharose with Protein G as ligand. Protein G has high affinity for the Fc region of IgG antibodies.

应用

Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target Proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with Protein G as ligand. Protein G has high affinity for the Fc region of IgG antibodies from a broad variety of species. There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis. Protein G Mag Sepharose combines well-proven enrichment methods with the magnetic beads platform, which has excellent properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

特点和优势

  • Visible and dense Sepharose based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
  • Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
  • Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

Sepharose is a trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

储存分类代码

3 - Flammable liquids

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

实验方案

Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.

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