ligand
concanavalin A (con A)
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 28-9520-85
storage condition
(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol), (20% Ethanol containing 0.1 M Acetate Buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl2)
parameter
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
4-9
working range
4-6
storage temp.
2-8°C
General description
HiTrap® Con A 4B is a ready-to-use column prepacked with Con A Sepharose™ 4B, a medium for convenient separation and purification of glycoproteins, polysaccharides, and glycolipids.
Application
Concanavalin A (Con A) is a tetrameric metalloProtein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose™ is routinely used for separation and purification of glycoProteins, polysaccharides, and glycolipids.
Features and Benefits
- Con A Sepharose™ is concanavalin A coupled to Sepharose™ 4B.
- HiTrap® Con A 4B columns can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as ÄKTA® design.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
ÄKTA is a registered trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
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signalword
Warning
hcodes
存储类别
3 - Flammable liquids