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Merck
CN

GE28-9520-85

HiTrap® Con A 4B

Cytiva 28-9520-85, pack of 5 × 1 mL

别名:

chromatography column, concanavalin A column, glycolipids affinity column, glycoprotein affinity column, prepacked column

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NACRES:
NA.56
UNSPSC Code:
23151816

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ligand

concanavalin A (con A)

packaging

pack of 5 × 1 mL

manufacturer/tradename

Cytiva 28-9520-85

storage condition

(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol), (20% Ethanol containing 0.1 M Acetate Buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl2)

parameter

42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

4-9

working range

4-6

storage temp.

2-8°C

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General description

HiTrap® Con A 4B is a ready-to-use column prepacked with Con A Sepharose™ 4B, a medium for convenient separation and purification of glycoproteins, polysaccharides, and glycolipids.

Application

Concanavalin A (Con A) is a tetrameric metalloProtein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose™ is routinely used for separation and purification of glycoProteins, polysaccharides, and glycolipids.

Features and Benefits

  • Con A Sepharose™ is concanavalin A coupled to Sepharose™ 4B.
  • HiTrap® Con A 4B columns can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as ÄKTA® design.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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分析证书(COA)

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商品

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

实验方案

Purification or Removal of Glycoproteins and Polysaccharides

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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