跳转至内容
Merck
CN

GE28-9523-60

GST GraviTrap™

Cytiva 28-9523-60

别名:

GST columns, affinity columns, chromatography columns, glutathione Sepharose 4B, gravity columns

登录 查看组织和合同定价。

选择尺寸

关于此项目

NACRES:
NA.56
UNSPSC Code:
41106501

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

ligand

glutathione

packaging

pkg of 10 ea

manufacturer/tradename

Cytiva 28-9523-60

bed volume

2 mL

column I.D.

15 mm

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

4-13

working range

4-13

capacity

≥20 mg binding capacity(recombinant glutathione S-transferase (rGST)), >25 mg binding capacity (horse liver GST/ml medium)

相关类别

General description

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST GraviTrap™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose™ 4B, sufficient for purification of up to 20 mg of fusion protein.

Application

GST GraviTrap™ contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose™ 4B, sufficient for purification of up to 20 mg of fusion Protein. GST-tagged Proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of prepacked GST GraviTrap™ columns, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged Proteins.

Features and Benefits

  • Convenient, prepacked gravity-fLow columns.
  • Allows direct purification of both clarified and unclarified cell lysates.
  • Yields up to 20 mg of pure Proteins per column.
  • Yields up to 20 mg of pure Proteins per column

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

法规信息

新产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助，请联系 客户支持

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

商品

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

Troubleshooting Strategies in GST-tagged Protein Purification

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

实验方案

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持