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Merck
CN

GE28-9537-67

HiTrap® TALON® Crude

Cytiva 28-9537-67, pack of 5 × 5 mL

别名:

Crude TALON Column, HiTrap TALON, TALON Crude Column

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NACRES:
NA.56
UNSPSC Code:
23151816

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technique(s)

liquid chromatography (LC): suitable

separation technique

affinity

storage temp.

2-8°C

form

prepacked column

crosslinking

cross-linked

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 28-9537-67

storage condition

(20% Ethanol)

parameter

0.5 Mpa max. pressure, 20 mL/min max. flow rate, 5 mL/min flow rate

bed volume

5 mL

column I.D. × L

16 mm × 25 mm

matrix

6% cross-linked agarose

matrix active group

cobalt

particle size

60-160 μm

cleaning

2-14(Co2+ stripped medium)

operating pH

3-12(Co2+ stripped medium)

capacity

≤20 mg binding capacity (histidine-tagged protein / mL medium)

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General description

HiTrap® Talon® crude 1 mL and 5 mL columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media. Talon® Superflow and prepacked HiTrap® Talon® crude columns allow protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

HiTrap® Talon® crude columns allow for rapid separations and easy scale-up. Samples can be applied directly to the column after thorough cell disruption - without precentrifugation and filtration. The filters in the top and bottom of the column make it possible to directly load unclarified lysates on the column without causing back pressure problems or leakage of the Talon® Superflow beads. Prior to loading samples on HiTrap® Talon® crude columns, cell lysate should be prepared using methods such as sonication, homogenization, or freeze/thaw.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Features and Benefits

  • Columns prepacked with cobalt-based chromatography media designed for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).
  • Suitable for IMAC purification of histidine-tagged proteins when Ni2+ is not the optimal choice of metal ion.
  • Compatible with commonly used IMAC reagents and appropriate for purifying proteins under native or denaturing conditions.
  • Prepacked HiTrap® Talon® crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of proteins.
  • HiTrap® Talon® crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Talon is a registered trademark of Clontech

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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Lot/Batch Number

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商品

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

实验方案

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

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