ligand
protein G
包装
pack of 2 × 1 mL
制造商/商品名称
Cytiva 28-9670-66
储存条件
(20% Ethanol, 10% medium slurry)
参数
Room temperature temp. range
基质
paramagnetic, spherical, highly cross-linked agarose particles
容量
27 mg binding capacity(human IgG/ml gel)
储存温度
2-8°C
一般描述
protein G Mag Sepharose™ Xtra magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants. The high capacity, > 27 mg human IgG /mL medium allow for the efficient capture of the antibody with high yield and purity. Approximately 350 μg purified human IgG can be obtained from each purification run.
应用
Protein G Mag Sepharose™ Xtra magnetic beads combine proven affinity chromatography methods with the magnetic bead platform to improve simplification and ease-of-use in sample preparation. Mag Sepharose™ beads are dense, black and visible, and this makes them simple to handle. Less smearing effects and aggregate formations of the magnetic beads mean that no detergents are needed in the buffers.
Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device. Protein G Mag Sepharose™ Xtra is available in a 2 × 1 mL pack for 20 purifications and in a 5 × 1 mL pack for 50 purifications. The product is based on Sepharose™ medium with magnetite incorporated and the ligand is native Protein G. Protein G has high affinity for the Fc region of IgG antibody from a variety of species.
Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device. Protein G Mag Sepharose™ Xtra is available in a 2 × 1 mL pack for 20 purifications and in a 5 × 1 mL pack for 50 purifications. The product is based on Sepharose™ medium with magnetite incorporated and the ligand is native Protein G. Protein G has high affinity for the Fc region of IgG antibody from a variety of species.
特点和优势
- Efficient, high capacity small-scale purification and screening of antibodies from various species; 27 μg human IgG/μL magnetic beads
- High purity and yield
- Robust parallel screening of antibodies with high reproducibility
- Simple capture of antibodies from small or large sample volumes (low-microliter to high-milliliter scale)
- Visible and dense Sepharose™ based magnetic beads give increased simplicity for handling
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents.
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
Sepharose is a trademark of Cytiva
警示用语:
Warning
危险声明
储存分类代码
3 - Flammable liquids
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