ligand
Cibacron Blue
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9782-43
storage condition
(20% Ethanol, 0.1 M potassium phosphate), (20% Ethanol, 0.9% NaCl, 1 mM Ca2₊ and 1 mM Mn2₊)
parameter
1.5 bar (22 psi) (Over the Packed Bed During Operation), 22 psi
bed size
7.7 mm × 100 mm
bed volume
4.7 mL
column I.D.
7.7 mm
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
3-13
working range
4-12
suitability
suitable for bioprocess medium
storage temp.
2-8°C
General description
HiScreen™ columns prepacked with Blue Sepharose™ Fast FLow are an excellent choice for method optimization and parameter screening for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons, and related proteins.
Application
HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
HiScreen™ Blue FF is prepacked with the BioProcess chromatography medium Blue Sepharose™ 6 Fast Flow. This medium has Cibacron Blue 3G covalently attached to the Sepharose™ 6 Fast FLow matrix by the triazine coupling method. The Blue dye binds many Proteins, such as albumin, interferon, lipoProteins, and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors (e.g., NAD).
HiScreen™ Blue FF is prepacked with the BioProcess chromatography medium Blue Sepharose™ 6 Fast Flow. This medium has Cibacron Blue 3G covalently attached to the Sepharose™ 6 Fast FLow matrix by the triazine coupling method. The Blue dye binds many Proteins, such as albumin, interferon, lipoProteins, and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors (e.g., NAD).
Features and Benefits
- Can be used for depletion of albumin.
- Prepacked, ready-to-use columns for convenient process development.
- Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
- Easy connection in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
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Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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商品
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
实验方案
This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.
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