material
bed support (polyamide), glass column, polypropylene fiber (reinforced glass)
mol wt
<10000 Da (globular proteins)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9893-31
parameter
<1.0 mL/min flow rate, <5 mL sample volume, 0.3 Mpa (42 psi)
bed volume
120 mL
column L × I.D.
60 cm × 16 mm
matrix
agarose dextran composite, dextran-agarose particle platform, polymeric
matrix active group
dextran-agarose phase
avg. part. size
34 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
General description
HiLoad® 16/600 Superdex® 30 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography separating peptides.
Application
All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
Superdex® prep grade (pg) is produced by covalent bonding of Dextran to highly cross-linked Agarose.The separation properties of these media are determined by the Dextran component. Steep selectivity curves give excellent resolving power for peptides and Proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
for high-resolution preparative gel filtration chromatography separating peptides
Features and Benefits
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to ÄKTA® design systems.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
