material
bed support (polyamide), fiberglass end pieces (polypropylene reinforced), glass column
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 10-600 kDa (fractionation range), Mr 1-100 kDa (dextrans fractionation range)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9893-35
storage condition
(20% Ethanol )
parameter
<5 mL sample volume, 0.3 Mpa max. pressure, 0.3-1.6 mL/min flow rate (recommended)
technique(s)
liquid chromatography (LC): suitable
bed volume
120 mL
column L × I.D.
60 cm × 16 mm
matrix
cross-linked agaorse, spherical, dextran-agarose particle platform
matrix active group
agarose, dextran-agarose phase
avg. part. size
34 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
General description
Superdex®制备级(pg)是通过葡聚糖与高度交联琼脂糖的共价结合产生的。这些介质的分离性质由葡聚糖组分决定。陡峭的选择性曲线为分子量范围内的多肽和蛋白质提供了优良的分辨能力：Mr < 10 000 (Superdex® 30 pg)、Mr 3 000至70 000 (Superdex® 75 pg)以及Mr 10 000 600 000 (Superdex® 200 pg)。该培养基结合了高机械强度和高亲水性，允许高流速和极低的非特异性相互作用。
Application
Features and Benefits
- 分辨率高，运行时间短，回收率良好。
- 高流量下的高容量。
- 分离可以扩展至生产水平。
- 预先包装实现了方便和再现性。
- 1/16”配件方便连接到AKTA设计系统。
Preparation Note
Analysis Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.
This page shows how to refold proteins using gel filtration and products from Cytiva.
商品
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
相关内容
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| GE28-9893-35 | 04061838551696 |