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Merck
CN

GE28-9893-35

HiLoad® 16/600 Superdex® 200 pg

Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

别名:

16/600 Superdex 200, HiLoad Superdex 200

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关于此项目

NACRES:
SB.52
UNSPSC Code:
41115700
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产品名称

HiLoad® 16/600 Superdex® 200 pg, Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

material

bed support (polyamide)
fiberglass end pieces (polypropylene reinforced)
glass column

form

prepacked column

crosslinking

cross-linked

mol wt

protein Mr 10-600 kDa (fractionation range)
Mr 1-100 kDa (dextrans fractionation range)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9893-35

storage condition

(20% Ethanol )

parameter

<5 mL sample volume
0.3 Mpa max. pressure
0.3-1.6 mL/min flow rate (recommended)

technique(s)

liquid chromatography (LC): suitable

bed volume

120 mL

column L × I.D.

60 cm × 16 mm

matrix

cross-linked agaorse, spherical
dextran-agarose particle platform

matrix active group

agarose
dextran-agarose phase

avg. part. size

34 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

Preparation Note

储存于4至30 °C(20%乙醇)。
请注意,该产品发货时可能距离到期仅剩 90 天。 有关特定批次的有效期的更多信息,请联系技术服务部门。
Storage Temp.: 4°C to 30°C

Legal Information

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

General description

HiLoad® 16/600 Superdex® 200制备级XK柱设计用于高分辨率制备凝胶过滤色谱。该色谱柱非常适合分离单克隆抗体与二聚体和污染物。

Superdex®制备级(pg)是通过葡聚糖与高度交联琼脂糖的共价结合产生的。这些介质的分离性质由葡聚糖组分决定。陡峭的选择性曲线为分子量范围内的多肽和蛋白质提供了优良的分辨能力:Mr < 10 000 (Superdex® 30 pg)、Mr 3 000至70 000 (Superdex® 75 pg)以及Mr 10 000 600 000 (Superdex® 200 pg)。该培养基结合了高机械强度和高亲水性,允许高流速和极低的非特异性相互作用。

Analysis Note

如需查看本品的分析证书(COA),请访问www.cytiva.com

Application

所有常用缓冲液,1 M NaOH、0.1 M HCl、1 M乙酸、8 M尿素、6 M 盐酸胍、1 % SDS、70 %乙醇、30 %异丙醇、30 %乙腈。
用于高分辨率制备凝胶过滤色谱,例如分离mAbs与二聚体和污染物

Features and Benefits

  • 分辨率高,运行时间短,回收率良好。
  • 高流量下的高容量。
  • 分离可以扩展至生产水平。
  • 预先包装实现了方便和再现性。
  • 1/16”配件方便连接到AKTA设计系统。

Other Notes

探索最适于 HPLCLC-MS 分析的LiChropur试剂

pictograms

Flame

signalword

Warning

hcodes

存储类别

3 - Flammable liquids


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

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Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

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相关内容

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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