material
bed support (polyamide), fiberglass end pieces (polypropylene reinforced), glass column
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 10-600 kDa (fractionation range), Mr 1-100 kDa (dextrans fractionation range)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9893-36
storage condition
(20% Ethanol )
parameter
≤13 mL sample volume, 0.3 Mpa, 0.9-4.4 mL/min flow rate
technique(s)
liquid chromatography (LC): suitable
bed volume
320 mL
column L × I.D.
60 cm × 26 mm
matrix
cross-linked agarose, spherical, dextran-agarose particle platform
matrix active group
agarose, dextran-agarose phase
avg. part. size
34 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
General description
Superdex ®制备级（pg）是由葡聚糖与高度交联的琼脂糖共价键合而成。这些介质的分离特性由葡聚糖成分决定。陡峭的选择性曲线为以下分子量范围内的肽和蛋白质提供出色的分辨能力：Mr <10 000 (Superdex®30 pg)、Mr 3 000 至 70 000 (Superdex® 75 pg) 和 Mr 10 000 600 000 (Superdex ®200 pg)。该介质结合了高机械强度和高亲水性，可实现高流速和最小的非特异性相互作用。
Application
Features and Benefits
- 分辨率高，运行时间短，回收率好。
- 高流速下的高容量。
- 分离可以扩大到生产规模。
- 预填充柱实现方便性和重现性。
- 1/16” 接头方便连接到 AKTA 设计系统。
Preparation Note
Analysis Note
Legal Information
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
商品
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
实验方案
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.
Cytiva的Superdex是由葡聚糖和琼脂糖的复合基础基质组成的SEC介质。本页面介绍如何使用superdex执行分离任务。
相关内容
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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