ligand
butyl
packaging
pack of 1 column
manufacturer/tradename
Cytiva 28-9924-73
bed size
7.7 mm × 100 mm
column I.D.
7.7 mm
matrix
highly cross-linked agarose
avg. part. size
75 μm (d50v)
cleaning
3-14
working range
3-13
capacity
27 mg binding capacity(27 mg BSA /ml (at QB10%))
suitability
suitable for bioprocess medium
General description
Capto™ Phenyl (High sub) and Capto™ Butyl are hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification.
Application
HiScreen™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resins) and designed for method optimization and parameter screening. HiScreen™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.
Capto™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose™ 4 Fast Flow. Higher flow rates can be used with Capto™ Butyl during equilibration, wash and regeneration.
Capto™ Butyl is intended for initial and intermediate step purifications requiring a chromatography medium with Low to medium hydrophobicity. Capto™ Butyl often works efficiently with rather Low salt concentrations. The mechanism of binding and elution onto the butyl ligand is different from that onto the phenyl ligand, giving a different selectivity. Performance during sample run and selectivity are similar to that of Butyl Sepharose™ 4 Fast Flow. Higher flow rates can be used with Capto™ Butyl during equilibration, wash and regeneration.
Features and Benefits
- Prepacked with Capto™ Butyl
- Excellent for method optimization and parameter screening due to the 10 cm bed height
- Easily connected in series to achieve 20 cm bed height
- Small bed volume for fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same flow velocity
Preparation Note
Store at 4 to 30 °C (20% Ethanol)
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
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