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TALON® Superflow Cobalt-IMAC
manufacturer/tradename
Cytiva 29-0005-93
packaging
pkg of 50 x 100 μL
bed volume
100 μL
matrix
6% cross-linked agarose
particle size
60-160 μm
cleaning
2-14(Co2+-stripped medium)
working range
3-12(Co2+-stripped medium)
capacity
≤1 mg binding capacity(histidine-tagged), ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
storage temp.
2-8°C
General description
His SpinTrap™ Talon® is a single-use column for rapid screening with small samples of histidine-tagged proteins.
Application
His SpinTrap™ Talon® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap™ Talon® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from Talon® Superflow medium is negligible.
Features and Benefits
- Suitable for purification of multiple samples in parallel, such as screening.
- Convenient, ready-to-use single-use spin column, prepacked with 100 μL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
- With a microcentrifuge, one run takes about 10 minutes.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
SpinTrap is a trademark of Cytiva
Talon is a registered trademark of Clontech
signalword
Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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商品
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
实验方案
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
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