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Merck
CN

GE29-0005-93

His Spintrap™ TALON®

Cytiva 29-0005-93

别名:

HIS spin columns, HIS-tagged purification columns, IMAC columns, TALON Superflow cobalt-IMAC, immobilized metal ion chromatography columns

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UNSPSC代码:
41106500
NACRES:
NA.56

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TALON® Superflow Cobalt-IMAC

制造商/商品名称

Cytiva 29-0005-93

包装

pkg of 50 x 100 μL

柱床体积

100 μL

基质

6% cross-linked agarose

粒径

60-160 μm

cleaning

2-14(Co2+-stripped medium)

工作范围

3-12(Co2+-stripped medium)

容量

≤1 mg binding capacity(histidine-tagged)
 ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

储存温度

2-8°C

相关类别

一般描述

His SpinTrap™ Talon® is a single-use column for rapid screening with small samples of histidine-tagged proteins.

应用

His SpinTrap™ Talon® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap™ Talon® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from Talon® Superflow medium is negligible.

特点和优势

  • Suitable for purification of multiple samples in parallel, such as screening.
  • Convenient, ready-to-use single-use spin column, prepacked with 100 μL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
  • With a microcentrifuge, one run takes about 10 minutes.

制备说明

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律信息

SpinTrap is a trademark of Cytiva
Talon is a registered trademark of Clontech

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

法规信息

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分析证书(COA)

Lot/Batch Number

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商品

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

实验方案

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

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