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GE29-0005-94

His GraviTrap™ TALON®

Cytiva 29-0005-94, pack of 10 columns

别名:

His GraviTrap

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NACRES:
NA.56
UNSPSC Code:
23151816

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产品名称

His GraviTrap™ TALON®, Cytiva 29-0005-94, pack of 10 columns

ligand

TALON® Superflow Cobalt-IMAC

packaging

pack of 10 columns

manufacturer/tradename

Cytiva 29-0005-94

bed volume

1 mL

column I.D.

15 mm

matrix

6% cross-linked agarose

particle size

60-160 μm

cleaning

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

capacity

≤15 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
 ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

storage temp.

2-8°C

相关类别

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

His GraviTrap™ Talon® gravity flow columns are designed for convenient and efficient gravity flow purification of histidine-tagged Proteins directly from clarified or unclarified cell lysates. His GraviTrap™ Talon® columns are delivered in a package that can be converted into a column stand to simplify purification, eliminating the need for additional equipment to complete the protocol. Special column frits protect the chromatography medium from running dry during purification.

Large sample volumes can be applied in one go, and the histidine-tagged Protein is effectively eluted in a small volume. LabMate PD-10 Buffer Reservoir can be connected to the columns for handling of sample volumes above 10 ml. Leakage of cobalt ions from Talon® Superflow medium is negligible.

Features and Benefits

  • Convenient histidine-tagged purification with high purity (>90%) and binding capacity of up to 15 mg/column.
  • Ready-to-use gravity flow column prepacked with 1 mL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
  • One run takes about 30 minutes, and large sample volumes can be applied all at once.

General description

His GraviTrap™ Talon® is a prepacked gravity f low column for convenient manual purification of histidine-tagged proteins.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

GraviTrap is a trademark of Cytiva
Talon is a registered trademark of Clontech

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Warning

hcodes

H226

存储类别

3 - Flammable liquids

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商品

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns

His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.

实验方案

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

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