form
prepacked column
crosslinking
cross-linked
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0485-76
storage condition
(20% ethanol)
parameter
0.5 Mpa max. pressure, 1 mL/min flow rate, 4 mL/min max. flow rate
technique(s)
liquid chromatography (LC): suitable
bed volume
1 mL
column I.D. × L
7 mm × 25 mm
matrix
highly cross-linked agarose, spherical
matrix active group
Protein A
avg. part. size
34 μm
cleaning in place
2-10
working range
3-9
capacity
~20 mg binding capacity (human IgG / mL resin)
separation technique
affinity
storage temp.
2-8°C
General description
HiTrap Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.
Application
HiTrap Protein A HP is a prepacked Protein A Sepharose High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.
Features and Benefits
- High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
- 34 μm bead size for high-performance, high-resolution purifications.
- Designed for use with syringe, pump, or chromatography system such as ÄKTA design or FPLC System.
- Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
- Robust, easy-to-use method.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Legal Information
HiTrap is a registered trademark of Cytiva
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signalword
Warning
hcodes
存储类别
3 - Flammable liquids