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Merck
CN

GE29-0485-86

HisTrap™ Excel

Cytiva 29-0485-86, pack of 1 mL

别名:

HIS chromatography column, HIS-tagged purification columns, IMAC column, Ni Sepharose excel, immobilized metal ion chromatography column, prepacked column

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NACRES:
NA.56
UNSPSC Code:
23151816

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产品名称

HisTrap™ Excel, Cytiva 29-0485-86, pack of 1 mL

form

prepacked column

crosslinking

cross-linked

packaging

pack of 1 mL

manufacturer/tradename

Cytiva 29-0485-86

storage condition

(20% ethanol)

parameter

1-4 mL/min flow rate
5 bar max. pressure

technique(s)

liquid chromatography (LC): suitable

bed volume

1 mL

column I.D. × L

7 mm × 25 mm

matrix

highly cross-linked agarose, spherical

matrix active group

Nickel

average diameter

90 μm

cleaning

2-14

working range

2-12

capacity

≥10 mg binding capacity (histidine-tagged protein / mL sedimented medium)

separation technique

affinity

shipped in

ambient

storage temp.

2-30°C

相关类别

Features and Benefits

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • HisTrap excel columns allow direct purification of cell-free, unclarified samples
  • HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet.
  • HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.

General description

Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.
HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). HisTrap excel 1 mL and 5 mL are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the resin enables fast and convenient purifications. The special type of filter in the top and bottom of the columns makes it possible to load large volumes of cell-free, unclarified samples directly on the columns without causing back pressure problems. This time-saving property helps prevent degradation and loss of sensitive target proteins.

Other Notes

H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Legal Information

HisTrap is a trademark of Cytiva

pictograms

Environment
GHS09

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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商品

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

实验方案

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using HisTrap™ Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from Cytiva.

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose® Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

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