form
prepacked column
crosslinking
cross-linked
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0486-31
storage condition
(20% ethanol)
parameter
1 mL/min flow rate, 4 mL/min max. flow rate, 5 bar max. pressure
technique(s)
liquid chromatography (LC): suitable
bed volume
1 mL
column I.D. × L
7 mm × 25 mm
matrix
6% cross-linked agarose
matrix active group
Ni2+ ions
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
~40 mg binding capacity (histidine-tagged protein / mL medium)
separation technique
affinity
shipped in
ambient
storage temp.
2-30°C
General description
HisTrap FF crude columns offer the convenience of simple, one-step purification of histidine-tagged proteins directly from homogenized, unclarified cell lysate by immobilized metal ion affinity chromatography (IMAC).
Application
HisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. This prepacked column is intended for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). After thorough cell disruption, it is possible to load the unclarified lysate on the column without precentrifugation and filtration of the sample. Extending the duration of the mechanical treatment of the sample to ensure a more complete lysis is recommended. For optimal results, we recommend first addition of lysozyme and DNase I followed by a mechanical lysis, for example by sonication. The homogenized sample can then be loaded directly on the column without a clarification step which may prevent degradation of the target protein and increase the activity.
Features and Benefits
- Optimized for purification of histidine-tagged proteins directly from homogenized, unclarified cell lysates.
- High binding capacity, approx. 40 mg/ml medium and negligible Ni2+ leakage
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Reduced sample preparation time, which minimizes risk of protein degradation by proteases.
- No need for centrifugation or filtration of the samples. Direct sample application.
- Reliable purification of histidine-tagged proteins directly from unclarified lysates–simply sonicate and run.
- Reduced sample preparation time, which minimizes risk of protein degradation by proteases
- Simple operation with a syringe, pump, or chromatography system such as ÄKTAdesign or FPLC System
- Permit rapid yet reliable separations with a minimum of sample preparation and equipment
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HisTrap is a trademark of Cytiva
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signalword
Warning
hcodes
存储类别
3 - Flammable liquids
flash_point_f
Not applicable
flash_point_c
Not applicable