parameter
5 bar
column I.D.
7 mm
matrix
rigid, highly cross-linked agarose
avg. part. size
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
working range
2-10(pH interval where the medium can be operated without significant change in function.)
suitability
suitable for bioprocess medium
storage temp.
2-8°C
ligand
recombinant protein L (E. coli), mammalian free
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0486-65
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General description
HiTrap™ Protein L columns are prepacked with Capto™ L affinity chromatography resin for purification of antibody fragments containing the kappa light chain. This resin combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has a strong affinity to the variable region of antibody kappa light chains.
Application
Affinity chromatoghraphy; capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs.
Features and Benefits
- Suitable for the capture of a wide range of antibody fragments such as antigen-binding fragments (Fabs), domain antibodies (dAbs), and single-chain variable fragments (scFvs).
- Enables a platform approach to antibody fragment purification.
- High binding capacity, low ligand leakage, and selectivity for a broad range of antibody fragments reduces process time and amount of resin.
- Convenient for process development, as well as small-scale purification.
- HiTrap™ format enables easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA™ system.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Legal Information
HisTrap is a trademark of Cytiva
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Warning
hcodes
存储类别
3 - Flammable liquids
法规信息
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Philipp A Kronenberg et al.
Frontiers in cellular and infection microbiology, 13, 1162530-1162530 (2023-04-04)
Alveolar (AE) and cystic echinococcosis (CE) are severe parasitic zoonoses caused by the larval stages of Echinococcus multilocularis and E. granulosus sensu lato, respectively. A panel of 7 monoclonal antibodies (mAbs) was selected against major diagnostic epitopes of both species.
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