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Merck
CN

GE29-0513-25

HiTrap® Q HP

Cytiva 29-0513-25, pack of 1 mL

别名:

IEX column, Q Sepharose HP, chromatography cartridge, cytiva 29051325, ion exchange resin, prepacked column, strong anion exchanger

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NACRES:
NA.56
UNSPSC Code:
23151816

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产品名称

HiTrap® Q HP, Cytiva 29-0513-25, pack of 1 mL

ligand

quaternary amine

description

Ion Exchanger Type (value)

packaging

pack of 1 mL

manufacturer/tradename

Cytiva 29-0513-25

availability

not available in North America

parameter

42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning

1-14

working range

2-12

suitability

suitable for bioprocess medium

相关类别

Application

Separation of protein using a strong anion exchange medium prepacked in the HiTrap column format.

Features and Benefits

  • 34 μm bead size for high-performance, high-resolution purifications.
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
  • Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
  • Strong quaternary ammonium (Q) anion exchanger.

General description

HiTrap Q HP are prepacked, ready-to-use Q Sepharose High Performance strong anion exchange columns for high-resolution, small-scale protein purification.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Legal Information

HiTrap is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

存储类别

3 - Flammable liquids

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商品

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

实验方案

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

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