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Merck
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GE29-0915-96

Sigma-Aldrich

Superose® 6 Increase 10/300 GL

Cytiva 29-0915-96, 10/300 GL

别名:

Superose® 6 Increase

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UNSPSC代码:
41115700
NACRES:
SB.52

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物料

glass column
polyethylene filter

产品线

Superose®

表单

prepacked column

交联

cross-linked

分子量

5000-5,000,000 Da (globular proteins)

包装

package of 1 ea

制造商/商品名称

Cytiva 29-0915-96

储存条件

(20% ethanol )

参数

<0.35 mL/min flow rate
<0.75 mL/min flow rate (20% ethanol at room temp)
<0.75 mL/min flow rate
<1.50 mL/min flow rate (water at room temp)
25-500 μL sample volume
3 Mpa

技术

liquid chromatography (LC): suitable

柱床体积

24 mL

基质

cross-linked agarose
polymeric

基质活性基团

agarose phase

粒径

~ 8.6 µm (d50v) (d50v)

cleaning

1-14

工作范围

3-12

分离技术

size exclusion (SEC)

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相关类别

一般描述

Superose® 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale (< 500 μl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000.

应用

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)
Superose® 6 Increase 10/300 GL column is suitable for small-scale (25 – 500 μl sample volume) preparative purification for obtaining milligram amounts of highly size-homogenous target proteins at the polishing stage of a purification procedure. It is also well-suited for the characterization of large protein complexes and for the study of protein-protein interactions.

特点和优势

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

制备说明

Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

法律信息

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

储存分类代码

3 - Flammable liquids

法规信息

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分析证书(COA)

Lot/Batch Number

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Anja Fux et al.
Cell chemical biology, 26(1), 48-59 (2018-11-13)
Detection of dynamic protein-protein interactions within complexes and networks remains a challenging task. Here, we show by the example of the proteolytic ClpXP complex the utility of combined chemical cross-linking and mass spectrometry (XL-MS) to map interactions within ClpP and

商品

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

实验方案

Performing a Separation with Superose

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

相关内容

Separation Options with Superose®

Separation options for Size Exclusion Chromatography with Superose from Cytiva.

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