material
glass column, polyethylene filter
product line
Superose®
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 40 MDa (exclusion limit), protein Mr 5-5000 kDa (fractionation range), Mp 1-300 kDa (dextrans fractionation range)
packaging
package of 1 ea
manufacturer/tradename
Cytiva 29-0915-96
storage condition
(20% ethanol )
parameter
0.5 mL/min flow rate (recommended), 1.50 mL/min max. flow rate, 25-500 μL sample volume, 3 Mpa pressure drop required
technique(s)
liquid chromatography (LC): suitable
L × I.D.
300 mm × 10 mm
bed volume
24 mL
matrix
cross-linked agarose
matrix active group
agarose
particle size
~ 8.6 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
General description
Superose® 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale (< 500 μl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000.
Application
Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)
Superose® 6 Increase 10/300 GL column is suitable for small-scale (25 – 500 μl sample volume) preparative purification for obtaining milligram amounts of highly size-homogenous target proteins at the polishing stage of a purification procedure. It is also well-suited for the characterization of large protein complexes and for the study of protein-protein interactions.
Features and Benefits
- Increased resolution compared with Superose 6, for higher purity and improved analysis results.
- Reduced runtime compared with Superose 6, for rapid results.
- Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
- Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.
Preparation Note
Storage Temp.: 4°C to 30°C
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva
存储类别
3 - Flammable liquids