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Merck
CN

GE29-0915-98

Sigma-Aldrich

Superose® 6 Increase

3.2/300, Cytiva 29-0915-98, 8.6 μm avg. part. size

别名:

Size exclusion chromatography, Superose 6 column

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UNSPSC代码:
41115700
NACRES:
SB.52

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物料

glass column

产品线

Superose®

表单

prepacked column

交联

cross-linked

分子量

protein Mr 40 MDa (exclusion limit)
protein Mr 5-5000 kDa (fractionation range)
Mp 1-300 kDa (dextrans fractionation range)

包装

pkg of 1 ea

制造商/商品名称

Cytiva 29-0915-98

储存条件

(20% ethanol )

参数

0.04 mL/min flow rate (recommended)
0.15 mL/min max. flow rate
2 Mpa pressure drop required
4-50 μL sample volume

技术

liquid chromatography (LC): suitable

bed diam. × H

3.2 mm × 300 mm

柱床体积

2.4 mL

基质

cross-linked agarose

基质活性基团

agarose

平均零件尺寸

8.6 μm

cleaning

1-14

工作范围

3-12

分离技术

size exclusion (SEC)

运输

ambient

储存温度

2-30°C

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相关类别

一般描述

Superose® 6 Increase 3.2/300 is a versatile, prepacked column for size exclusion chromatography in small-scale preparative purification (4 to 50 μl sample volume), as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. When high resolution is required, sample amount is limited and low buffer consumption is important, this is the column of choice.

应用

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)

特点和优势

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

制备说明

Storage Temp.: 4°C to 30°C
Store at 4 to 30 °C (20% Ethanol)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

法律信息

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

储存分类代码

3 - Flammable liquids

法规信息

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商品

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

实验方案

Performing a Separation with Superose

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

相关内容

Separation Options with Superose®

Separation options for Size Exclusion Chromatography with Superose from Cytiva.

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