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Merck
CN

GE29-0915-98

Superose® 6 Increase

3.2/300, Cytiva 29-0915-98, 8.6 μm avg. part. size

别名:

Size exclusion chromatography, Superose 6 column

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NACRES:
SB.52
UNSPSC Code:
41115700

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material

glass column

product line

Superose®

form

prepacked column

crosslinking

cross-linked

mol wt

protein Mr 40 MDa (exclusion limit), protein Mr 5-5000 kDa (fractionation range), Mp 1-300 kDa (dextrans fractionation range)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 29-0915-98

storage condition

(20% ethanol )

parameter

0.04 mL/min flow rate (recommended), 0.15 mL/min max. flow rate, 2 Mpa pressure drop required, 4-50 μL sample volume

technique(s)

liquid chromatography (LC): suitable

bed diam. × H

3.2 mm × 300 mm

bed volume

2.4 mL

matrix

cross-linked agarose

matrix active group

agarose

avg. part. size

8.6 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

shipped in

ambient

storage temp.

2-30°C

相关类别

General description

Superose® 6 Increase 3.2/300 is a versatile, prepacked column for size exclusion chromatography in small-scale preparative purification (4 to 50 μl sample volume), as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. When high resolution is required, sample amount is limited and low buffer consumption is important, this is the column of choice.

Application

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)

Features and Benefits

  • Increased resolution compared with Superose 6, for higher purity and improved analysis results.
  • Reduced runtime compared with Superose 6, for rapid results.
  • Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
  • Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

Preparation Note

Storage Temp.: 4°C to 30°C
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva
Superose is a registered trademark of Cytiva

存储类别

3 - Flammable liquids

法规信息

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商品

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

实验方案

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Performing a Separation with Superose

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

相关内容

Separation Options with Superose®

Separation options for Size Exclusion Chromatography with Superose from Cytiva.

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