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Merck
CN

GEE70073X

Exonuclease I, 10 U/μL

Cytiva E70073X, pack of 5000 units

别名:

Exonuclease I Enzyme, ExoI, PCR cleanup enzyme, single-stranded DNA exonuclease

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NACRES:
NA.53
UNSPSC Code:
12352204
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产品名称

Exonuclease I, 10 U/μL, Cytiva E70073X, pack of 5000 units

biological source

Escherichia coli

packaging

pack of 5000 units

manufacturer/tradename

Cytiva E70073X

shipped in

dry ice

storage temp.

−20°C

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Exonuclease I has been used to quantify the expression of 56 genes in each F2 XY gonad.

Biochem/physiol Actions

Exonuclease I (Exo I) from E. coli can degrade single-stranded DNA (ssDNA) from 3′ to 5′. It participates in various DNA repair systems, like, RecBCD-dependent homologous recombination, methyl-dependent mismatch repair and genomic tandem repeat maintenance.

Features and Benefits

  • Elimination of residual single-stranded DNA containing a 3′-terminus.
  • Measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA.
  • Measuring DNA helicase activity.

General description

Acts specifically on single-stranded DNA degrading it processively in the 3′- to 5′-direction, producing 5′-mononucleotides.
Exonuclease I (Exo I) from E. coli is an Mg2+ ion dependent exonuclease.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

存储类别

12 - Non Combustible Liquids

法规信息

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历史批次信息供参考:

分析证书(COA)

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Structural and Biochemical Studies of a Moderately Thermophilic Exonuclease I from Methylocaldum szegediense
Fei L, et al.
Testing, 10(2), e0117470-e0117470 (2015)
Elucidation of the transcription network governing mammalian sex determination by exploiting strain-specific susceptibility to sex reversal
Munger S C, et al.
Genes & Development, 23(21), 2521-2536 (2009)

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