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Merck
CN

GEE79000Z

DNA Polymerase, Thermo Sequenase

10,000 Units, Cytiva E79000Z

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UNSPSC代码:
41106314
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特点

10,000 Units

制造商/商品名称

Cytiva E79000Z

运输

dry ice

储存温度

−20°C

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一般描述

Thermo Sequenase™ DNA polymerase is a novel thermostable DNA polymerase that uses ddNTPs as readily as dNTP substrates at temperatures similar to those used in standard PCR. ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing. Each nucleotide base of that particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation. This property, in addition to thermostability, makes Thermo Sequenase™ DNA polymerase an excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase. The properties of Thermo Sequenase™ also enable its use in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed.

特点和优势

  • Uniform and easy-to-read band patterns.
  • Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences
  • Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing

危险声明

预防措施声明

储存分类代码

12 - Non Combustible Liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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分析证书(COA)

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