packaging
pkg of 1 kit
manufacturer/tradename
Cytiva RPN2235
General description
Amersham™ ECL™ Select: Western blotting detection reagent with highly intense signal output, for detection of medium to very low protein levels.
Amersham™ ECL™ Select is a high sensitivity reagent for chemiluminescent Western blotting detection. High signal intensity makes Amersham™ ECL™ Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. Its long lasting signals allow for multiple exposures and a convenient time window between experiment and analysis. Signals can be detected with CCD camera or X-ray film.
Amersham™ ECL™ Select typically exhibits a dynamic range that is complementary to Amersham™ ECL™ Prime, and similar to Amersham™ ECL™ Advance.
Amersham™ ECL™ Select is a high sensitivity reagent for chemiluminescent Western blotting detection. High signal intensity makes Amersham™ ECL™ Select suitable for the most demanding Western blotting applications, allowing for detection of minute protein quantities. Its long lasting signals allow for multiple exposures and a convenient time window between experiment and analysis. Signals can be detected with CCD camera or X-ray film.
Amersham™ ECL™ Select typically exhibits a dynamic range that is complementary to Amersham™ ECL™ Prime, and similar to Amersham™ ECL™ Advance.
Application
Western Blotting
Features and Benefits
- Improved sensitivity: Produces a very high signal intensity
- Quantitative measurements: Linear dynamic range allowing for quantitation of medium to very low abundant proteins
- Works with a wide range of antibody dilutions, reducing the need for lengthy optimization
- Optimized for imaging with ImageQuant LAS 4000 (CCD-based imaging) and compatible with Amersham™ Hyperfilm
- Compatible with Rainbow™ Molecular Weight Markers and Amersham™ ECL™ DualVue Western Blotting Markers.
- Optimized for use with Amersham™ Hybond-P (PVDF) membranes and compatible with Amersham™ Hybond® ECL™ (nitrocellulose) membranes
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
includes
Solution A (Luminol solution, 50 mL)
Solution B (Peroxide solution, 50 mL)
sufficient for 1000 cm2 membrane
complete packsize
for 1000 cm2 membrane
Solution A (Luminol solution, 50 mL)
Solution B (Peroxide solution, 50 mL)
sufficient for 1000 cm2 membrane
complete packsize
for 1000 cm2 membrane
Legal Information
Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva
Rainbow is a trademark of Cytiva
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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This page describes troubleshooting strategies in the detection of GST-tagged proteins.
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
描述被分子生物学家用于检测转印膜上蛋白质和核酸的方法的背景和实验方案。
实验方案
This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.
This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.
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